iBodies technology

Polymer-based antibody mimetics: inhibitors + antibody

iBodies®, the novel macromolecules designed to fully substitute antibodies in common biochemical methods, are now ready for routine use in laboratories. iBodies® were successfully tested in ELISA, flow cytometry, confocal microscopy, Western Blotting, protein immobilization and immunoprecipitation methods. These fully synthetic antibody mimetics consist of N-(2-hydroxypropyl) methacrylamide (HPMA) copolymer backbone decorated with low-molecular-weight functional groups: a targeting ligand, an affinity anchor and an imaging probe (Fig.1). In theory, iBodies® can be custom designed towards any protein of interest (provided a ligand is known) and tailored based on the intended applications.


               Fig.1: Schematic structure of iBody


iBodies vs. Antibodies


Current stage of development enables the use of iBodies® primarily in the scientific research in the above mentioned applications where specific antibodies against proteins  are commonly used (confocal microscopy, flow cytometry, immunoprecipitation, ELISA etc.). The anti-His-tag iBody, representing our most-developed iBody, is an excellent alternative for antibodies used in western blotting (Fig.2).

Fig.2: Detection of 10xHis and 6xHis-tagged proteins on western blot. Comparison of detection with commercially available mAbs.

IP owners & patent status

iBodies® is a registered trade mark of IOCB Tech s.r.o.

Institute of Organic Chemistry and Biochemistry, the Czech Academy of Sciences, Prague
Institute of Macromolecular Chemistry, the Czech Academy of Sciences, Prague
Charles University, Prague
Patent applications: PV 2015-19, PV 2015-20, PCT: CZ201605002, CZ201605003



iBodies: Modular Synthetic Antibody Mimetics Based on Hydrophilic Polymers Decorated with Functional Moieties.

Šácha P, Knedlík T, Schimer J, et al.  Angew Chem Int Ed Engl. 2016;55(7):2356-60.

Inhibitor-Decorated Polymer Conjugates Targeting Fibroblast Activation Protein

Petra Dvořáková, Petr Bušek, Tomáš Knedlík, et al.  Journal of Medicinal Chemistry 2017 60 (20), 8385-8393

Identification of Protein Targets of Bioactive Small Molecules Using Randomly Photomodified Probes

Petr Šimon, Tomáš Knedlík, Kristýna Blažková, et al.  ACS Chemical Biology 2018 13 (12), 3333-334