Products

iBody anti-polyHis 488 Targeting Variably Long Polyhistidine Fusion Tags

designed for fluorescent detection at 500 nm

Anti-polyHis 488 iBody is able to specifically target and bind polyhistidne fusion tags. The so called His-tag, commonly engineered to recombinant proteins, is the most frequently used affinity tag utilized for purification, detection and characterization of such molecules. The ligand used for specific targeting is the tris-trinitriloacetic acid (tris-NTA) which is able to bind the polyhistidine sequence through the formation of a stable complex via a metal cation mediator. iBody anti-polyHis 488 is also accessorized with ATTO 488 fluorophore, which serves as a tool for facile and fast detection. Furthermore, biotin is conjugated to the polymer carrier. It assists in immobilization of the protein of interest and potentially in additional detection with chemiluminesce.

  • Properties
  • Downloads

Application

anti-polyHis iBodies serve as a replacement for monoclonal anti-His antibodies in a variety of biochemical applications such as western blotting (WB), ELISA, flow cytometry, confocal fluorescent microscopy, protein immobilization on resin etc.

Product Description

Targeted biomolecule specification: Protein containing polyhistidine sequence (6×His, 10×His - the most widely used purification tags) fused to the N- or C-terminus of a protein of interest.

 

 

Targeting ligand: tris-NTA (tris-nitrilotriacetic acid) charged with cobalt cations

 

Affinity anchor: biotin

Characteristics

Mr ~ 99 kDa; ATTO 488 fluorophore (7 wt%; 10 molecules), biotin content (8 wt%; 27 molecules), tris-NTA ligand (16 wt%; 12 molecules).

iBody KD = 4 nM (10xHis-protein as a model system; measured with surface plasmon resonance) 

Storage

The iBody is stable at room temperature. For long term storage, place the solution to -20°C.

Reference

Angew Chem Int Edit 2016, 55, 2356-2360