Products

iBody Directed towards Glutamate Carboxypeptidase II

anti-GCPII iBody

Anti-GCPII iBody is able to specifically target and bind glutamate carboxypeptidase II, a transmembrane metallopeptidase that is strongly expressed in prostate carcinoma. The ligand used for specific targeting is GCPII inhibitor which binds the enzyme with extremely high affinities. Anti-GCPII iBody is also accessorized with ATTO 488 fluorophore, which serves as a tool for facile detection and visualization of the protein. Furthermore, biotin, which assists in immobilization of the protein of interest and potentially in additional detection with chemiluminesce, is conjugated to the polymer carrier.

  • Properties

Application

anti-GCPII iBodies serve as a replacement for monoclonal anti-GCPII antibodies in a variety of biochemical applications such as immunoprecipitation, protein immobilization, ELISA, confocal microscopy, cytometry etc. iBodies bind GCPII with extremely high affinity with KDs in picomolar range.

EXAMPLE: 

Fig.1: iBody live cell imaging:  Visualization of cells expressing GCPII (GCPII+) and with anti-GCPII iBody. Negative controls (NC): NC GCPII- = cells not expressing GCPII, NC iBody = iBody lacking GCPII inhibitor

Product Description

Targeted biomolecule specification: 
Glutamate carboxypeptidase (GCP) II, also known as Prostate-Specific Membrane Antigen (PSMA) is a membrane metallopeptidase expressed in the brain. GCPII is overexpressed in prostate cancer and tumor vasculature.

Targeting ligand: tight‐binding GCPII inhibitor

 

Fig. 2: Chemical structure of the GCPII inhibior

Affinity anchor: biotin

Characteristics

iBody KD (GCPII) = 4 pM; verified by SPR (KD < 20 pM) 

Storage

The iBody is stable at room temperature. For long term storage, place the solution to -20°C. 

Reference

Angew Chem Int Edit 2016, 55, 2356-2360