Products

iBody Directed towards Glutathione S-transferase Tag

anti-GST tag iBodies

Anti-GST tag iBody is able to specifically target and bind glutathione S-transferase tag. The so called GST-tag, commonly engineered to recombinant proteins, is a frequently used affinity tag utilized for purification, detection and characterization of such molecules. The ligand used for specific targeting is glutathione which displays a strong binding affinity for GST. iBody also carries biotin anchor which assists in immobilization of the protein of interest and potentially in additional detection with chemiluminesce. iBody can be additionally accessorized with a fluorophore of choice.

  • Properties

Application

anti-GST tag iBodies® serve as a replacement for monoclonal anti-GST antibodies in a variety of biochemical applications such as immunoprecipitation, protein immobilization on resin etc.

EXAMPLE:

Fig 1: iBody pull-down from cell lysate: Isolation of GST-tagged capsid protein from HIV-1 virus using anti‑GST‑tag iBody bound to streptavidin agarose resin

Negative controls (NC): NC iBody = iBody lacking glutathione, NC streptavidin = no iBody used

Product Description

Targeted biomolecule specification: Fusion proteins with glutathione S-transferase (GST) tag.

Targeting ligand: glutathione

 

Fig. 2: Chemical structure of glutathione (GHT)

Affinity anchor: biotin

Characteristics

iBody KD = 4 nM (GST-capsid protein as a model system; measured with surface plasmon resonance)

Storage

The iBody is stable at room temperature. For long term storage, place the solution to -20°C.