iBody Directed towards Glutathione S-transferase Tag
anti-GST tag iBodies
Anti-GST tag iBody is able to specifically target and bind glutathione S-transferase tag. The so called GST-tag, commonly engineered to recombinant proteins, is a frequently used affinity tag utilized for purification, detection and characterization of such molecules. The ligand used for specific targeting is glutathione which displays a strong binding affinity for GST. iBody also carries biotin anchor which assists in immobilization of the protein of interest and potentially in additional detection with chemiluminesce. iBody can be additionally accessorized with a fluorophore of choice.
Application
anti-GST tag iBodies® serve as a replacement for monoclonal anti-GST antibodies in a variety of biochemical applications such as immunoprecipitation, protein immobilization on resin etc.
EXAMPLE:
Fig 1: iBody pull-down from cell lysate: Isolation of GST-tagged capsid protein from HIV-1 virus using anti‑GST‑tag iBody bound to streptavidin agarose resin
Negative controls (NC): NC iBody = iBody lacking glutathione, NC streptavidin = no iBody used
Product Description
Targeted biomolecule specification: Fusion proteins with glutathione S-transferase (GST) tag.
Targeting ligand: glutathione
Fig. 2: Chemical structure of glutathione (GHT)
Affinity anchor: biotin
Characteristics
iBody KD = 4 nM (GST-capsid protein as a model system; measured with surface plasmon resonance)
Storage
The iBody is stable at room temperature. For long term storage, place the solution to -20°C.