iBody Directed towards Human Immunodeficiency Virus-1 Protease
anti-HIV-1 protease iBodies
Anti-HIV-1 protease iBody is able to specifically target and bind human immunodeficiency virus-1 protease, an aspartic protease, which is crucial in the life cycle of HIV; the well known retrovirus causing AIDS. The ligand used for specific targeting is ritonavir inhibitor which binds the HIV-1 protease with high affinities. Anti-HIV 1 protease iBody is also accessorized with ATTO 488 fluorophore, which serves as a tool for facile detection and visualization of the protein. Furthermore, biotin, which assists in immobilization of the protein of interest and potentially in additional detection with chemiluminesce, is conjugated to the polymer carrier.
anti-HIV 1 protease iBodies serve as a replacement for monoclonal antibodies in a variety of biochemical applications such as immunoprecipitation, protein immobilization, ELISA, confocal microscopy, cytometry etc.
EXAMPLE: Fig. 1: iBody pull-down from cell lysate: Isolation of HIV-1 protease from HIV-1 protease-spiked LNCaP cell lysate using anti-HIV-protease iBody (=iBody pull-down) and anti-Aspartic proteases iBody (=Non-specific iBody). Negative control (NC): NC iBody = iBody lacking HIV-1 protease inhibitor
Targeted biomolecule specification:
HIV-1 protease is an aspartic protease that is crucial for the life-cycle of HIV, the retrovirus causing AIDS.
Targeting ligand: ritonavir based HIV-1 protease inhibitor
Fig. 2: Chemical structure of ritonavir
Affinity anchor: biotin
iBody KD = 8 nM (HIV-1 protease as a model system; measured with surface plasmon resonance)
The iBody is stable at room temperature. For long term storage, place the solution to -20°C.
Angew Chem Int Edit 2016, 55, 2356-2360